R5279

Anti-Rab9 from rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Ras-Associated Protein Rab9, Anti-Ras-Related Protein Rab9
• MDL number: MFCD03097193
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~23 kDa
• species reactivity: rat, mouse, human
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH3T3 cells fixed and permeabilized with 3% paraformaldehyde followed by 0.4% saponin; western blot: 5-10 μg/mL using whole extract of human A431 cells
• UniProt accession no.: P51151
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAB9A(9367) ; mouse ... Rab9(56382) ; rat ... Rab9a(84589)

Description

General description

Rab9 is a member of the Rab family of guanosine triphosphatases (GTPases). Rab9 GTPase is localized predominantly to late endosomes. Rab proteins contain conserved regions involved in guanine nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif implicated in subcellular targeting.

Immunogen

synthetic peptide corresponding to amino acid residues 188-200 of human Rab9, conjugated to KLH.

Application

Anti-Rab9 from rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the trans-Golgi network (TGN). Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein TIP47 (tail-interacting protein of 47 kDa). This interaction is required for Rab9 stability on late endosomes. Anti-Rab9 antibodies may be used as a marker for late endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable