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Polyethylene glycol solution

Name: Polyethylene glycol solution
Brand: SIGMA

Hybri-Max^™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

Synonym(s):

PEG Solution, Polyethylene Glycol

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About This Item

MDL number:

MFCD00081839

UNSPSC Code:

12161902

PubChem Substance ID:

24898829

NACRES:

NA.75

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Properties

grade

Hybri-Max^™

Quality Segment

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

mol wt

average mol wt 1,450

concentration

50 % (w/v)

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

Description

General description

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Application

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Polyethylene glycol solution has been used in the hybridization of the following:

irradiated camel fibroblast cells with A23 hamster cells
mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
rabbit splenocytes and cell line 240E-1

Biochem/physiol Actions

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Packaging

Packaged in sealed ampules under nitrogen.

Preparation Note

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Show Differences

1 of 1

This Item	P7306	P1458	76293
Sigma-Aldrich P7181 Polyethylene glycol solution	Sigma-Aldrich P7306 Polyethylene glycol/ dimethyl sulfoxide solution	Sigma-Aldrich P1458 Polyethylene glycol solution	Sigma-Aldrich 76293 Polyethylene glycol solution
form solution	form solution	form -	form liquid
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
mol wt average mol wt 1,450	mol wt average mol wt 1,450	mol wt average mol wt 8,000	mol wt -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -
impurities endotoxin, tested	impurities endotoxin, tested	impurities -	impurities DNases, none detected, RNases, none detected, insoluble matter, none detected, phosphatases, none detected, proteases, none detected
concentration 50 % (w/v)	concentration 50 % (w/v)	concentration 40 % (w/w) in H₂O	concentration ~50% in H₂O

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

212.0 °F - closed cup

flash_point_c

100 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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