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Proteinase from Aspergillus melleus

Name: Proteinase from <I>Aspergillus melleus</I>
Brand: SIGMA

Type XXIII, ≥3 units/mg solid

Synonym(s):

Protease from Aspergillus melleus, Proteinase from Aspergillus sp.

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About This Item

CAS Number:

9001-92-7

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-642-4

NACRES:

NA.54

MDL number:

MFCD00132092

Specific activity:

≥3 units/mg solid

Biological source:

Aspergillus sp. (A. melleus)

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Properties

biological source

Aspergillus sp. (A. melleus)

Quality Segment

200

type

Type XXIII

form

solid

specific activity

≥3 units/mg solid

storage temp.

2-8°C

Description

Application

Proteinase is an enzyme used to break down proteins by hydrolyzing peptide bonds. Proteinase is used to degrade proteins, to study proteinase inhibitors and to study thermal inactivation kinetics. Proteinase is used in nucleic acid isolation procedures in incubations. It is used to study proteinase-activated receptors, such as the transducers of proteinase-mediated signaling in inflammation and the immune response. Product P4032 is from Aspergillus melleus and has been used to non-specifically degraded xylanase from Streptomyces halstedii.

Biochem/physiol Actions

Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

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This Item	86247	P8038	G5160
Sigma-Aldrich P4032 Proteinase from Aspergillus melleus	Sigma-Aldrich 86247 Taka-Diastase from Aspergillus oryzae	Sigma-Aldrich P8038 Proteinase, bacterial	Sigma-Aldrich G5160 β-Galactosidase from Aspergillus oryzae
description Type XXIII, ≥3 units/mg solid	description powder, slightly beige, ~100 U/mg	description Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder	description ≥8.0 units/mg solid
specific activity ≥3 units/mg solid	specific activity ~100 U/mg	specific activity ≤5.0 Kunitz units/mg solid DNase, ≤0.05 Kunitz units/mg solid RNase, 7.0-14.0 units/mg solid	specific activity ≥8.0 units/mg solid
biological source Aspergillus sp. (A. melleus)	biological source Aspergillus sp. (A. oryzae)	biological source -	biological source -
form solid	form powder	form lyophilized powder	form solid
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
type Type XXIII	type -	type Type XXIV	type -

Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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