N2250

Naphthol AS-BI phosphate disodium salt hydrate

fluorogenic, ≥96.0% (HPLC), powder

• Synonym(s): 2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate
• Empirical Formula (Hill Notation): C₁₈H₁₃BrNNa₂O₆P · xH₂O
• CAS Number: 207569-04-8
• Molecular Weight: 496.16 (anhydrous basis)
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897558
• NACRES: NA.83

Properties

• product name: Naphthol AS-BI phosphate disodium salt hydrate, ≥96.0% (HPLC)
• Quality Level: 200
• Assay: ≥96.0% (HPLC)
• form: powder
• solubility: water: 50 mg/mL, clear, colorless to yellow
• storage temp.: −20°C
• SMILES string: O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O
• InChI: 1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2
• InChI key: WNPKMQCBZKBNOS-UHFFFAOYSA-L

Description

General description

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.

Application

Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:

• in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
• as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
• to perform alkaline phosphatase reaction

Biochem/physiol Actions

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Substrates

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves