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MAK128

Sigma-Aldrich

Indican Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Indoxyl Sulfate Assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

storage temp.

−20°C

General description

Indican (indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of tryptophan in the small intestine. Indican is water soluble and excreted through urine. Increases in urine indican can indicate protein maldigestion or malabsortion, or the overgrowth of gut anaerobic bacteria. Indican levels in urine can also increase in response to a high protein diet.

Application

Indican Assay Kit has been used to measure indican concentration in urine samples.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

The Indican Assay kit provides a simple and direct procedure for measuring indican levels in urine.

Principle

This assay is based on an improved Curzon and Walsh method in which the reaction of indican with p-dimethylaminobenzaldehyde results in a colorimetric product measured at 480 nm. This assay has a linear detection range between 0.2-20 mg/dL in the 96 well plate assay.

Pictograms

CorrosionExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8B - Non-combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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