MAK107

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

• Synonym(s): Lecithin:cholesterol acyltransferase activity assay kit
• EC Number: 200-659-6
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• application(s): pharmaceutical
• detection method: fluorometric
• relevant disease(s): cardiovascular diseases
• storage temp.: −20°C
• Gene Information: human ... LCAT(3931)

Description

General description

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Application

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.

LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for measuring phospholipase activity of LCAT in plasma or serum.

Principle

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λ_Ex=390 nm/λ_Em=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Kit Components Only

• Substrate Reagent .1 mL
• READ Reagent 30 mL
• LCAT Assay Buffer 20 mL

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225,H301 + H311 + H331,H370
• Precautionary Statements: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 49.5 °F
• Flash Point(C): 9.7 °C