M7569

Monoclonal Anti-MTA2 antibody produced in mouse

clone MTA2-276, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Metasisis-associated Gene
• MDL number: MFCD03097137
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MTA2-276, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~73 kDa
• species reactivity: mouse, human, bovine, rat, hamster, canine, monkey
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using 293T total cell extract
• isotype: IgG1
• UniProt accession no.: O94776
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MTA2(9219) ; mouse ... Mta2(23942) ; rat ... Mta2(361724)

Description

General description

Metastasis associated 1 family member 2 (MTA2) is part of the nucleosome remodeling and histone deacetylation complex. This 668 amino acid protein possesses four different domains.

Monoclonal Anti-MTA2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MTA2, conjugated to KLH. Human metastasis-associated protein 2 (MTA2) shares 58% homology to human MTA1 and MTA3 proteins, the C-terminus being more divergent than the N-terminus. MTA2 comprises N-terminal bromo-adjacent homology (BAH) and MTA1 homology 2 (ELM2) domain, SANTand zinc finger (ZnF) domain. It also has the nuclear localization region at C-terminus.

Immunogen

synthetic peptide corresponding to amino acids 626-641of human MTA2, conjugated to KLH.

Application

Monoclonal Anti-MTA2 antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunocytochemistry
• Western blotting
• antibody microarray

Biochem/physiol Actions

Human metastasis-associated protein 2 (MTA2) interacts with the histone deacetylase-1 HDAC1 and within the nuclear remodeling and deacetylation complexes ATP-dependent helicase -nucleosome remodelling and histone deacetylation (Mi2/NuRD), suggests that these proteins are involved in transcriptional repression. In the p53 pathway, MTA2/MTA1L1 is better known as protein in the deacetylase complexes p53 target protein in the deacetylase complexes (PID). It inhibits p53-dependent transcriptional activation and its growth arrest and apoptosis functionality. showing that deacetylation and functional interaction by the PID/MTA2/MTA1L1 associated nucleosome remodeling deacetylase (NuRD) complex may represent an important pathway to regulated p53 function.

Metastasis associated 1 family member 2 (MTA2) has been associated with tumor progression and metastasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves