M6435

Methionine Aminopeptidase from Pyrococcus furiosus

≥93% (SDS-PAGE), recombinant, expressed in E. coli

• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.4.11.18 (BRENDA, IUBMB)
• MDL number: MFCD03457470
• Specific activity: 0.5 units/mg protein
• Assay: ≥93% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥93% (SDS-PAGE)
• form: solution
• specific activity: 0.5 units/mg protein
• mol wt: 37 kDa by SDS-PAGE
• UniProt accession no.: P56218
• foreign activity: Other proteases, none detected
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: Pyrococcus furiosus DSM 3638 ... PF0541(1468383)

Description

General description

X-ray crystallography of the structure of methionine aminopeptidase from Pyrococcus furiosus or PfMAP was performed at a resolution of 1.75A and showed that the protein consists of a catalytic domain containing two cobalt ions in the active site and a unique insertion domain which is specific to the prokaryotic form of the protein.

Methionine aminopeptidase from Pyrococcus furiosus is a 32 kDa thermostable enzyme. It belongs to type 2a class of methionine aminopeptidase. Methionine aminopeptidase maintains protein homeostasis and coordinates posttranslational modification of proteins in eukaryotes.

Application

Methionine Aminopeptidase from Pyrococcus furiosus has been used in a study to analyze the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus. It has also been used in a study to examine the binding of a new class of pseudopeptide analog inhibitors.

Biochem/physiol Actions

Thermostable methionine aminopeptidase, which specifically liberates the N-terminal methioinine from proteins and peptides.

Physical form

Solution containing 0.01% Tween^® 20, 0.1 mM CoCl₂, and 10 mM Tris-HCl, pH 7.5.

Other Notes

One unit will hydrolyze 1 μmol of Met from Met-Pro-Ala-Ala-Gly in 1 minute at pH 7.5 at 37 °C.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable