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Hanks′ Balanced Salt Solution 10x

Name: Hanks′ Balanced Salt Solution 10x
Brand: SIGMA

Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Hanks’ Balanced Salt solution, HBSS

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Segment

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes

shipped in

ambient

storage temp.

room temp

Description

General description

Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salt Solution 10x has been used:

as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium[1]
to wash the biopsy specimens of lamina propria lymphocytes (LPLs)[2]
as a wash solution to wash the fish cell line during removal from growth medium[3]

Preparation Note

Supplement with 0.35 g/L sodium bicarbonate at 1X.

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1 of 1

This Item	H1641	H6648	H2387
Sigma-Aldrich H4641 Hanks′ Balanced Salt Solution 10x	Sigma-Aldrich H1641 Hanks′ Balanced Salt Solution 10x	Sigma-Aldrich H6648 Hanks′ Balanced Salt solution	Sigma-Aldrich H2387 Hanks′ Balanced Salts
form liquid	form liquid	form liquid	form powder
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components phenol red: yes	components -	components NaHCO₃: 0.35 g/L glucose: 1.0 g/L (Dextro) phenol red: no	components -
storage temp. room temp	storage temp. -	storage temp. room temp	storage temp. 2-8°C
shipped in ambient	shipped in -	shipped in ambient	shipped in -

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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