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Key Documents

GS59

Sigma-Aldrich

GST M5-5, Recombinant Human

Synonym(s):

glutathione S-transferase mu 5

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.55

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

>95% (SDS-PAGE)

form

frozen liquid

specific activity

183.9 units/mg protein

mol wt

27.4 kDa

concentration

3.2 mg/mL

storage temp.

−70°C

Gene Information

human ... GSTM5(2949)

General description

using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.

Biochem/physiol Actions

Glutathione S-transferase mu 5 (GSTM5) is an enzyme that in humans is encoded by the GSTM5 gene. Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
The GSTM5 gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual′s susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds.

Storage and Stability

The enzyme should be used by the end-user customer within 1 year of receipt.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Christian Delles et al.
Journal of hypertension, 26(7), 1343-1352 (2008-06-14)
Glutathione S-transferases are involved in defences against oxidative stress. We have recently demonstrated reduced expression of glutathione S-transferase mu type 1 (Gstm1) in a rat model of hypertension. Here, we examine the association between GSTM variants and hypertension in human.
Ann M Moyer et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 19(3), 811-821 (2010-03-05)
Lung cancer is commonly treated with platinum compounds. The "glutathione pathway" participates in the metabolism of platinum compounds. We set out to test the hypotheses that single nucleotide polymorphisms (SNPs) or copy number polymorphisms for genes within the glutathione pathway
Y V Patskovsky et al.
Biochemistry, 38(49), 16187-16194 (1999-12-10)
The hGSTM3 subunit, which is preferentially expressed in germ-line cells, has the greatest sequence divergence among the human mu class glutathione S-transferases. To determine a structural basis for the catalytic differences between hGSTM3-3 and other mu class enzymes, chimeric proteins
Carrie V Breton et al.
American journal of respiratory and critical care medicine, 179(7), 601-607 (2009-01-20)
The glutathione S-transferases (GSTs) are important detoxification enzymes. To investigate effects of variants in GST mu genes on lung function and assess their interactions with tobacco smoke exposure. In this prospective study, 14,836 lung function measurements were collected from 2,108
Ying Wang et al.
Journal of human genetics, 55(8), 490-494 (2010-05-21)
Coronary atherosclerosis is a complex and progressive condition that involves many biological pathways, including the oxidative stress and inflammatory response pathways. To investigate the association between common genetic variation within these two pathways and coronary atherosclerosis, we performed a comprehensive

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