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Key Documents

GE28-9889-38

Sigma-Aldrich

XK 16/40 Column

Synonym(s):

PCR Beads, XK Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

polypropylene column

packaging

1 ea of

Related Categories

General description

XK empty columns provide reliable and reproducible separations. The tube material is of borosilicate glass, which allows for visual inspection of the media bed. The columns are made of biocompatible materials that exhibit excellent chemical resistance. A broad range of column dimensions makes XK columns suitable for many different purification needs. Column packing can be performed using either a packing reservoir or an extra column tube attached with a packing connector. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments of the bed height, disassembly, and cleaning. The design of the adapter plunger gives a uniform flow, which maintains the integrity of the packed bed during operations. An extensive range of spare parts is available, facilitating maintenance.

Features and Benefits

Adapter QuickLock mechanism that facilitates column handling and cleaning
Cooling jacket for sensitive purifications
Fittings for direct connection to chromatographiy systems
Plunger design to ensure a homogeneous buffer distribution and reproducible packing
User friendly and robust empty columns, designed for liquid chromatography at low to medium pressure.

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Articles

Column Packing and Preparation for Affinity Chromatography of Antibodies

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Converting from Flow Velocity to Volumetric Flow Rates

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Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

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Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

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Puriļ¬cation of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni SepharoseĀ® Excel

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