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G4252

Sigma-Aldrich

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Synonym(s):

S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Grade IV

Quality Level

form

buffered aqueous glycerol solution

specific activity

≥400 units/mg protein

foreign activity

glyoxalase II ≤1%

storage temp.

2-8°C

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General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Unit Definition

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

Physical form

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

Analysis Note

Protein determined by biuret.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xuming Jia et al.
PloS one, 7(5), e36610-e36610 (2012-05-19)
Methylglyoxal (MG) is a highly reactive metabolite physiologically presented in all biological systems. The effects of MG on diabetes and hypertension have been long recognized. In the present study, we investigated the potential role of MG in obesity, one of
Megan Jack et al.
Translational research : the journal of laboratory and clinical medicine, 159(5), 355-365 (2012-04-17)
Diabetic neuropathy is the most common and debilitating complication of diabetes mellitus with more than half of all patients developing altered sensation as a result of damage to peripheral sensory neurons. Hyperglycemia results in altered nerve conduction velocities, loss of
On glyoxalase
H.D. Dakin & H.W. Dudley
The Journal of Biological Chemistry, 14, 423-431 (1913)
Víctor Cubillos et al.
Marine environmental research, 119, 59-71 (2016-05-28)
The estuarine slipper limpet Crepipatella dilatata is a gastropod that can survive prolonged periods of low salinities (< 24 PSU) caused by tidal changes and/or prolonged periods of rain. During low salinity events, C. dilatata can isolate its body from the outside
Ravi Gupta et al.
Journal of proteome research, 11(5), 2684-2696 (2012-04-11)
Plants' distribution and productivity are adversely affected by low temperature (LT) stress. LT induced proteins were analyzed by 2-DE-nano-LC-MS/MS in shoot secretome of Hippophae rhamnoides (seabuckthorn), a Himalayan wonder shrub. Seedlings were subjected to direct freezing stress (-5 °C), cold

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