F4383

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

≥98%

• Synonym(s): 4-Fluoro-7-sulfobenzofurazan ammonium salt, 7-Fluorobenz-2,1,3-oxadiazole-4-sulfonic acid ammonium salt, Ammonium 7-fluorobenzofurazan-4-sulfonate, SBD-F
• Linear Formula: C₆H₃FN₂O₄S · NH₃
• CAS Number: 84806-27-9
• Molecular Weight: 235.19
• UNSPSC Code: 12352106
• NACRES: NA.32
• PubChem Substance ID: 24894861
• MDL number: MFCD02682109
• Beilstein/REAXYS Number: 5199564
• Assay: ≥98%

Properties

• Quality Segment: 200
• assay: ≥98%
• storage condition: protect from light
• fluorescence: λ_ex 380 nm; λ_em 515 nm
• storage temp.: −20°C
• SMILES string: N.OS(=O)(=O)c1ccc(F)c2nonc12
• InChI: 1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3
• InChI key: JXLHNMVSKXFWAO-UHFFFAOYSA-N

Description

General description

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt, also known as SBD-F, is a low molecular weight sensitive fluorescent probe. SBD-F reacts with sulfhydryl groups (disulfides do not react with SBD-F and must first be reduced to thiols, such as tributylphosphine) to produce highly fluorescent compounds. The rate of reaction of thiols with SBD-F gradually increases with increasing pH. High fluorescence intensities are observed at pH 2-12. SBD-F has an excitation range between 380-385nm and an emission range between 510-515nm.

Application

Fluorescent probe for thiols and pre-column labeling of biological thiols for HPLC

SBD-F is suitable for the HPLC determination of biological thiols at the picomole level. SBD-F has been used in HPLC methods for measuring total plasma and serum homocysteine levels. It is also suitable to determine metallothionein in a tandem column HPLC method with an isocratic solvent system. SBD-F has been used for determining the position of disulfide linkages of cysteine residues in proteins and for detecting cysteine-containing peptides.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)