DFF100

DEAE–Sepharose^™

Name: DEAE–Sepharose™
Brand: SIGMA

Fast Flow

Synonym(s):

Diethylaminoethyl–Sepharose^™

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About This Item

CAS Number:

57407-08-6

UNSPSC Code:

47101511

NACRES:

NA.56

MDL number:

MFCD00146630

Key Documents

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Quality Level

200

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

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Show Differences

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This Item	A50120	DCL6B100	CCF100
Sigma-Aldrich DFF100 DEAE–Sepharose^™	A50120 DEAE–Sephadex^®	Sigma-Aldrich DCL6B100 DEAE–Sepharose^™	Sigma-Aldrich CCF100 CM Sepharose^™
pore size ~4,000,000 Da exclusion limit	pore size ~200,000 Da exclusion limit	pore size ~4,000,000 Da exclusion limit	pore size 4,000,000 exclusion limit (av. mol. wt.)
matrix 6% cross-linked agarose	matrix cross-linked dextran	matrix 6% cross-linked agarose	matrix 6% cross-linked agarose
technique(s) affinity chromatography: suitable	technique(s) protein purification: suitable	technique(s) affinity chromatography: suitable	technique(s) affinity chromatography: suitable
capacity 110-160 μeq/mL binding capacity(gel volume)	capacity 3-4 meq/g binding capacity	capacity 130-170 μeq/mL binding capacity (gel volume)(gel volume)	capacity 90-130 μeq/mL(gel basis)
form suspension	form powder	form suspension	form suspension
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose^™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose^™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

DEAE-Sepharose has been used in:

anion exchange chromatography
to screen polyisoprenyl phosphate phosphatase activity[1]
to purify isoinhibitors
for the purification of human immunodeficiency virus (HIV)
glycoprotein envelope (gp140 env)

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

comparable product

Product No.

Description

Pricing

GE17-0709-01

DEAE Sepharose^™ Fast Flow, Cytiva 17-0709-01, pack of 500 mL

pictograms

GHS02

signalword

Warning

hcodes

H226

pcodes

P210 - P233 - P240 - P241 - P242 - P243

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems

Rosenberg Y, et al.

Testing, 8(3), e58724-e58724 (2013)

Growth cone collapse and neurite retraction from cultured Helisoma neurons in response to antibody Fab fragments against an extracellular matrix protein.

Miller, J.D., et al.

Dev. Brain Res., 79(2), 203-218 (1994)

Adenovirus hexon protein is a potent adjuvant for activation of a cellular immune response.

Valérie Molinier-Frenkel et al.

Journal of virology, 76(1), 127-135 (2001-12-12)

The capacity of recombinant adenoviruses (rAd) to induce immunization against their transgene products has been well documented. In the present study, we evaluated the vaccinal adjuvant role of rAd independently of its vector function. BALB/c mice received one subcutaneous injection

Effective Purification Procedure of Aspergillus oryzae α−Amylase from Solid State Fermentation Cultures Including Concanavalin-A Sepharose

Terebiznik, M.R., et al.

Journal of Food Biochemistry, 19(5), 341-354 (1995)

Metals and activity of bovine heart cytochrome c oxidase are independent of polypeptide subunits III, VII, a, and b.

G L Yewey et al.

Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)

Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal

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Global Trade Item Number

SKU	GTIN
DFF100-100ML	04061833591550
DFF100-50ML	04061833591567

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DEAE–Sepharose™