CELLMM2

FLAG^® M Purification Kit

For Mammalian expression systems.

• Synonym(s): Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• Quality Segment: 200
• technique(s): protein extraction: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

The FLAG Purification Kit utilizes CelLytic^™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG^® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG^® application portal.

Features and Benefits

• Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
• ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
• Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Packaging

Sufficient for 3-5 uses of 1-ml affinity purification column.

Other Notes

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CelLytic is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

• C2978

  CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

  SDS
• SAE0194

  Purified 3xFLAG^® peptide, ≥95% (HPLC), lyophilized powder

  SDS
• A2220

  ANTI-FLAG^® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution

  SDS
• C2103

  Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.

  SDS

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3