The passage number for ECACC cell lines is lot specific and reported on the Certificate of Analysis. This document may be accessed directly at the supplier's website. Please see the link below to review the ECCAC product page:
https://www.culturecollections.org.uk/nop/product/u-87-mg
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About This Item
UNSPSC Code:
41106514
Biological source:
human brain (glioblastoma astrocytoma)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46
Morphology:
Epithelial-like
Manufacturer/tradename:
ECACC
Pricing and availability is not currently available.
biological source
human brain (glioblastoma astrocytoma)
manufacturer/tradename
ECACC
packaging
tube of 5 μg 89081402-DNA-5UG, pkg of vial of cells 89081402-1VL
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Neural studies
U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.
Biochem/physiol Actions
Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.
Human glioblastoma astrocytoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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This Item | |||
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| description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89081402, human brain (glioblastoma astrocytoma), Epithelial-like | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 86030402, human brain (astrocytoma), Glial | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92022711, human bone, Not specified | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012803, human breast (adenocarcinoma), Epithelial-like |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| relevant disease(s) cancer | relevant disease(s) cancer | relevant disease(s) cancer | relevant disease(s) cancer |
| growth mode Adherent | growth mode Adherent | growth mode Adherent | growth mode Adherent |
| packaging pkg of vial of cells 89081402-1VL, tube of 5 μg 89081402-DNA-5UG | packaging tube of 5 μg 86030402-DNA-5UG, vial of cells 86030402-1VL | packaging tube of 5 μg 92022711-DNA-5UG, pkg of vial of cells 92022711-1VL | packaging tube of 5 μg 86012803-DNA-5UG, pkg of vial of cells 86012803-1VL |
| manufacturer/tradename ECACC | manufacturer/tradename ECACC | manufacturer/tradename ECACC | manufacturer/tradename ECACC |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
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je voudrai connaitre le nombre de passage des deux lignées Pour la déclaration codecoh, j'ai besoin de votre numéro de DC ou AC ou IE, Merci
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Quante cellule per batch?
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This supplier cell bank, ECACC, states that adherent/attached cell line products are 2 - 3 x10^6 cells per vial, which would be the case for this product. Suspension cell lines from this supplier are 4 - 5 x10^6 cells per vial. They do not share 'batch' size information. For additional information please see the ECACC product page at the link : https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=89081402&collection=ecacc_gc
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