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B65

85042305, rat nerve, Neuronal

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About This Item

UNSPSC Code:
41106514

product name

B65, 85042305

biological source

rat nerve

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat nervous tissue neuronal

Cell Line Description

Ethyl nitrosourea induced tumour cell line. Neuronal cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Cell morphology significantly affected when grown in serum free media.

Application

Neurobiological studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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