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Carbonic Anhydrase from bovine erythrocytes

Name: Carbonic Anhydrase from bovine erythrocytes
Brand: SIGMA

For use as a marker in SDS-PAGE

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase

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About This Item

CAS Number:

9001-03-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-576-6

MDL number:

MFCD00081467

EC Number:

4.2.1.1 (BRENDA, IUBMB)

Biological source:

bovine erythrocytes

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Properties

biological source

bovine erythrocytes

Quality Segment

200

form

powder

mol wt

~29 kDa

packaging

vial of 5 mg

storage temp.

2-8°C

Description

General description

Carbonic Anhydrase is a metalloenzyme with zinc.[1] The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).[2]

Application

Carbonic Anhydrase has been used:

as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samples[3]
as a molecular marker in gel filtration chromatography[4]
as a standard protein to test the recovery of CMA/20 microdialysis probe[5]

Biochem/physiol Actions

Carbonic anhydrase is essential for the reversible hydration of carbon dioxide.[1] It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways.[1] Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.[2]

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C.

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This Item	C5024	C7025	C3666
Sigma-Aldrich C2273 Carbonic Anhydrase from bovine erythrocytes	Sigma-Aldrich C5024 Carbonic Anhydrase from bovine erythrocytes	Sigma-Aldrich C7025 Carbonic Anhydrase from bovine erythrocytes	Sigma-Aldrich C3666 Carbonic Anhydrase Isozyme II from bovine erythrocytes
biological source bovine erythrocytes	biological source -	biological source -	biological source -
form powder	form lyophilized powder	form powder	form powder
mol wt ~29 kDa	mol wt ~29 kDa	mol wt ~29,000	mol wt -
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
packaging vial of 5 mg	packaging -	packaging vial of 15 mg	packaging vial of ~1 mg protein