C0775

Crystal Violet

Dye content, ≥90%, certified by the Biological Stain Commission, powder

• Synonym(s): Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
• Linear Formula: C₂₅H₃₀N₃Cl
• CAS Number: 548-62-9
• Molecular Weight: 407.98
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 57653980
• EC Number: 208-953-6
• MDL number: MFCD00011750
• Beilstein/REAXYS Number: 3580948
• Colour Index Number: 42555

Properties

• Product Name: Crystal Violet, certified by the BSC
• agency: certified by the BSC
• Quality Segment: 200
• form: powder
• composition: Dye content, ≥90%
• technique(s): microbe id | staining: suitable
• color: green to very dark green
• pH: 2.5 — 3.5 (20 °C)
• mp: 205 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• density: 1.19 g/cm³ at 20 °C
• antibiotic activity spectrum: fungi
• application(s): diagnostic assay manufacturing; hematology; histology
• mode of action: cell membrane | interferes, enzyme | inhibits
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChI key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H318,H351,H410
• pcodes: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable