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A9171

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−AP

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About This Item

MDL number:
MFCD00162223
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:35,000
dot blot: 1:100,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Immunoglobulin Y (IgY) is also known as egg yolk immunoglobulin and is found mainly in birds, amphibians and reptiles. IgY cannot activate mammalian complement system and cannot bind to mammalian rheumatoid factors, and protein A or G. IgY facilitates an essential role in veterinary science, functional food and bio-products. It also has a crucial role in controlling infectious diseases of bacterial and viral origin.

Specificity

Anti-Chicken IgY (IgG) (whole molecule)-Alkaline Phosphatase antibody is specific for chicken IgY (IgG).

Immunogen

IgY (IgG) isolated from pooled normal chicken serum
IgY (IgG) isolated from pooled normal chicken serum.

Application

Anti-Chicken IgY (IgG) (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in:
  • immunoblotting
  • indirect enzyme linked immunosorbent assay (ELISA)
  • immunoglobulin assay
  • dot Blot
  • immunohistology

Biochem/physiol Actions

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yu-Chun Cai et al.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 27(6), 527-530 (2010-03-18)
Egg yolk immunoglobulin (IgY) is a major immunoglobulin from birds, amphibians and reptiles. IgY does not activate mammalian complement system, and does not bind to mammalian rheumatoid factors, protein A or G. In addition, it has a high yield, and
Wala Ahmad Amro et al.
Journal, genetic engineering & biotechnology, 16(1), 99-103 (2019-01-17)
The isolation of polyclonal antibodies from the serum of immunized mammals has significantly contributed to scientific research and diagnosis. The fact that recent technologies allow the production of antibodies in the yolk of eggs laid by hens, has led to
Effect of trypsin inhibitor activity in soya bean on growth performance, protein digestibility and incidence of sub-clinical necrotic enteritis in broiler chicken flocks
Palliyeguru MW, et al.
British Poultry Science, 52(3), 359-367 (2011)
Maternally derived egg hormones, antibodies and antimicrobial proteins: common and different pathways of maternal effects in Japanese quail
Okuliarova M, et al.
Testing, 9(11), e112817-e112817 (2014)
Johnny Lisboa et al.
mSphere, 6(1) (2021-02-05)
Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope. PG biogenesis is highly
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