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A2512

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonym(s):

Albumin from hen egg white, Ovalbumin

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About This Item

CAS Number:
UNSPSC Code:
12352202
eCl@ss:
42010201
EC Number:
232-692-7
NACRES:
NA.61
MDL number:
Biological source:
chicken egg white
Assay:
≥98% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
bioconjugation: suitable
Concentration:
4—20 mol/mol albumin
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biological source

chicken egg white

Quality Level

type

Grade VI

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

chromatography

concentration

4—20 mol/mol albumin

extent of labeling

4-20 mol mannose per mol ovalbumin protein

technique(s)

bioconjugation: suitable

solubility

H2O: soluble 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

UniProt accession no.

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

General description

Ovalbumin makes 75% of the total egg white protein.
Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.[1]

Application

A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.
Ovalbumin has been used:
  • as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil[2]
  • to induce allergic inflammation and atopic dermatitis in experimental mice
  • as a component in control mixture for validating tandem mass spectral analysis
  • as a model protein antigen to evaluate the in vivo efficacy of a chitosan/interleukin (IL)-12 adjuvant system

Biochem/physiol Actions

Albumin from chicken egg white serves as a natural adhesive.
Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C.[3] Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp, and 33 Glu which make it suitable for conjugation.

Physical form

Essentially salt free

Preparation Note

A further purification of A 5503 to reduce mannose content.

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This Item
A7641A5378A7642
assay

≥98% (agarose gel electrophoresis)

assay

≥95% (agarose gel electrophoresis)

assay

≥90% (agarose gel electrophoresis)

assay

-

technique(s)

bioconjugation: suitable

technique(s)

bioconjugation: suitable

technique(s)

bioconjugation: suitable

technique(s)

electrophoresis: suitable

biological source

chicken egg white

biological source

chicken egg white

biological source

chicken egg white

biological source

chicken egg white

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

Gene Information

chicken ... OVAL(396058)

Gene Information

chicken ... OVAL(396058)

Gene Information

chicken ... OVAL(396058)

Gene Information

chicken ... OVAL(396058)


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pictograms

Health hazard

signalword

Danger

hcodes

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hazard Classifications

Resp. Sens. 1



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Product Information Sheet


<BIG>Unger A, et al.</BIG>
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<BIG>Mielenz JR, et al.</BIG>
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PTP1B deficiency exacerbates inflammation and accelerates leukocyte trafficking in vivo
<BIG>Berdnikovs S, et al.</BIG>
Journal of Immunology, 188, 874-884 (2012)



Global Trade Item Number

SKUGTIN
A2512-250MG04061833350379
A2512-1G04061833350362
A2512-5G04061833350386

Questions

  1. How can we make 1 mm concentration of chicken egg albumin

    1 answer
    1. The molecular weight of this material is 44,287 daltons or g/mol. A 1 molar solution would be 44,287 grams per liter, a 100 mM solution would be 4,428 grams per liter, and so on. To prepare 100 ml of a 1 mM solution, 4.4287 g of the product will be needed.

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