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62310

Lipase from Rhizopus niveus

Name: Lipase from <I>Rhizopus niveus</I>
Brand: SIGMA

powder (fine), ≥1.5 U/mg

Synonym(s):

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:

9001-62-1

UNSPSC Code:

12352204

NACRES:

NA.26

EC Number:

232-619-9

EC Number:

3.1.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00131509

Specific activity:

≥1.5 U/mg

Biological source:

fungus (Rhizopus niveus)

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Properties

biological source

fungus (Rhizopus niveus)

Quality Segment

100

form

powder (fine)

specific activity

≥1.5 U/mg

mol wt

M_r ~83000

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Application

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Biochem/physiol Actions

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Other Notes

Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.