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51280

Hemin

BioXtra, from Porcine, ≥96.0% (HPLC)

Synonym(s):

Chloro(protoporphyrinato)iron(III), Chlorohemin, Chloroprotoporphyrin IX iron(III), Ferriprotoporphyrin IX chloride, Hemin(chloride)

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About This Item

Empirical Formula (Hill Notation):
C34H32ClFeN4O4
CAS Number:
16009-13-5
Molecular Weight:
651.94
UNSPSC Code:
12352202
NACRES:
NA.79
PubChem Substance ID:
57651319
EC Number:
240-140-1
Beilstein/REAXYS Number:
5229914
MDL number:
MFCD00010726
Form:
powder or crystals
Assay:
≥96.0% (HPLC)
Biological source:
Porcine
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biological source

Porcine

Quality Segment

200

product line

BioXtra

assay

≥96.0% (HPLC)

form

powder or crystals

technique(s)

tissue culture: suitable, transfection: suitable

storage temp.

2-8°C

SMILES string

Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C

InChI

1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;

InChI key

BTIJJDXEELBZFS-HXFTUNQESA-K

Application

Hemin is used for the following applications:
  • To study bacterial strains and growth conditions[1]
  • Used in short-term colony assays (the culture medium consists of hemin along with other components)[2]
  • Cell transfections[3]
It may be used in β-hematin formation assay for analyzing the inhibitory activity of MMV (Medicines for Malaria Venture) Malaria Box compounds against the formation of β-hematin.[4]

Biochem/physiol Actions

Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.

Hemin may be used to study the expression and represson of heme oxygenase 1 (HO-1) as well as the activity of HO-1. Hematin is the "X factor" required to grown Haemophilus influenzae.

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This Item
H90393741MBD0052
Hemin BioXtra, from Porcine, ≥96.0% (HPLC)

Sigma-Aldrich

51280

Hemin

Hemin from bovine, ≥90%

Sigma-Aldrich

H9039

Hemin

Hemin Chloride Iron-containing compound of porcine origin that can induce hydrogen peroxide-dependent lipid peroxidation. Reported to increase the number and size of myotubes and the frequency of myotube contractions.

Millipore

3741

Hemin Chloride

Hemin solution Ready-made microbial growth medium supplement, 0.5 g/L

Sigma-Aldrich

MBD0052

Hemin solution

biological source

Porcine

biological source

bovine

biological source

-

biological source

-

assay

≥96.0% (HPLC)

assay

≥90%

assay

-

assay

-

technique(s)

tissue culture: suitable, transfection: suitable

technique(s)

toxicology assay: suitable

technique(s)

-

technique(s)

-

form

powder or crystals

form

powder

form

solid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

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Questions

1–8 of 8 Questions  

  1. Can 51280 be used to induce the erythroid differentiation of the K562 cells? If yes, what would it need to be dissolved in to add be added to cell culture?

    1 answer

    1. This product is not tested for any specific application. However, the material has been used successfully by other researchers to induce erythroid differentiation and hemoglobinization of K562 cells. Please see the link below to review one such publication:
      https://pmc.ncbi.nlm.nih.gov/articles/PMC4665220/

      For culture use, this compound is typically prepared in DMSO at a concentration of 1 mM. The material is also soluble in 1.4 M ammonium hydroxide at 24 mg/mL at ambient temperature or 50 mg/ml when heated to 95°C for 5 minutes. Additionally, stock solutions of 0.25% hemin in 50% triethanolamine have been reported. Published methods suggest rates of 40 - 60 µM hemin for a treatment period of 1-5 days. Filter-sterilized stock solutions may be stored refrigerated, protected from light.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. What is the solubility of Hemin, product catalog # 51280, CAS 16009-13-5?

    1 answer

    1. The product with code 51280 can be dissolved in DMSO at a concentration of 1 mM. It is also soluble in 1.4 M ammonium hydroxide at a concentration of 50 mg/ml, and this can be achieved by heating the solution at 95°C for 5 minutes, resulting in a dark brown solution. Additionally, a stock solution of 0.25% hemin in 50% triethanolamine can be prepared. CAS number 16009-13-5, which corresponds to product 51280, is soluble in 1.4 M NaOH at a concentration of 25 mg/mL, and it is practically insoluble in water.

      Helpful?

  5. How can hemin be dissolved for cell culture experiments in macrophages? Is it necessary to couple it to serum/BSA?

    1 answer

    1. This product has not been tested for use in cell culture. However, a previously deleted product, H1652, has been tested. The H1652 was dissolved at 10 mg/mL in 1 M NaOH and added then added to the culture medium at 7 mg/L. This information would need to be validated for product 51280.

      Helpful?

  6. How stable are stock solutions of hemin (dissolved in 1N NaOH and diluted to 1 mM with dH2O)? Can they be stored at 4C or -20C?

    1 answer

    1. The solution stability has not been determined.

      Helpful?

  7. What is the solubility of Product 51280, Hemin?

    1 answer

    1. Hemin can be dissolved in DMSO at 1 mM. This product should be soluble in 1.4 M ammonium hydroxide (50 mg/ml) with 5 minutes of heating at 95 °C, yielding a dark brown solution.  A stock solution of 0.25% hemin in 50% triethanolamine can be made.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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