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E. coli ChromoSelect Agar B

NutriSelect® Plus, suitable for microbiology

Synonym(s):

E. coli Agar B ChromoSelect

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606
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sterility

non-sterile

Quality Segment

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , bile salt mixture, 1.5 g/L , casein enzymic hydrolysate, 20 g/L , X-glucuronide, 0.075 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

suitability

selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for coliforms

Application

E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.

Preparation Note

Suspend 36.6 g per litre in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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description

NutriSelect® Plus, suitable for microbiology

description

NutriSelect® Basic, powder, selective for Clostridium perfringens, pack of 250 g, suitable for microbiology

description

NutriSelect® Plus, powder, selective for coliforms, pack of 500 g, suitable for microbiology

description

NutriSelect® Plus, powder, for Escherichia coli, pkg of 100 g, pkg of 500 g

suitability

selective and differential for coliforms, selective and differential for Klebsiella spp., selective and differential for Escherichia coli

suitability

selective for Clostridium perfringens

suitability

selective and differential for Proteus spp., selective for coliforms, selective and differential for Escherichia coli, selective and differential for Salmonella spp.

suitability

selective and differential for coliforms, Escherichia coli

form

powder

form

powder

form

powder

form

powder

composition

agar, 15 g/L , casein enzymic hydrolysate, 20 g/L , X-glucuronide, 0.075 g/L , bile salt mixture, 1.5 g/L

composition

soy peptone, 5.0 g/L , sucrose, 3.0 g/L , ammonium iron(III) citrate, 0.2 g/L , tryptose (vegetable), 20.0 g/L , agar, 15.0 g/L , yeast extract, 15.0 g/L , L-cysteine hydrochloride, 1.0 g/L , tris buffer, 1.8 g/L , chromogenic mixture, 1.73 g/L , magnesiumsulfate heptahydrate, 0.1 g/L

composition

agar, 15 g/L , sodium chloride, 3.7 g/L , tryptose, 7.5 g/L , sodium sulfite, 1.6 g/L , sodium lauryl sulfate, 0.05 g/L , sodium deoxycholate, 0.1 g/L , di-potassium hydrogen phosphate, 3.3 g/L , lactose, 9.4 g/L , potassium hydrogen phosphate, 1 g/L , pararosanilin (fuchsin), 0.8 g/L , casein hydrolysate, 3.7 g/L , peptone from meat, 3.7 g/L , yeast extract, 1.2 g/L

composition

agar, 15 g/L , peptone, 20 g/L , bile salts, 1.5 g/L , X-β-D-glucuronide, 0.075 g/L

application(s)

microbiology, agriculture
bioburden testing
environmental
food and beverages
water monitoring

application(s)

microbiology, agriculture
bioburden testing
environmental
food and beverages
water monitoring

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology
water monitoring

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

final pH

7.2±0.2 (25 °C)

final pH

7.4±0.2 (25 °C)

final pH

7.2±0.2±25 °C

final pH

7.2±0.2 (25 °C in H2O)


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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