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4B200

Sepharose^™ 4B

Name: Sepharose<SUP>™</SUP> 4B
Brand: SIAL

45-165 μm bead diameter

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About This Item

CAS Number:

9012-36-6

UNSPSC Code:

12352200

PubChem Substance ID:

24873317

NACRES:

NA.21

MDL number:

MFCD08457579

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Properties

biological source

algae (marine)

Quality Segment

200

form

suspension

technique(s)

affinity chromatography: suitable

bead diameter

45-165 μm

pore size

30,000-50,000 Da fractionation range (Dextrans), 60,000-20,000,000 Da fractionation range (Globular proteins)

storage temp.

2-8°C

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

General description

Sepharose^™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Application

Sepharose^™ 4B has been used:

to conjugate scorpion venom to be used in affinity chromatography
for clearing of protein extracts prior to immunoprecipitation
for gel purification and desalting of phospholipid vesicles
for adsorption of immunoglobulin G (IgG)

Legal Information

Sepharose is a trademark of Cytiva

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Pictograms

GHS02

Signal Word

Warning

Hazard Codes

H226

Precautionary Statements

P210 - P233 - P240 - P241 - P242 - P243

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

100.4 - 109.4 °F - closed cup

Flash Point (°C)

38 - 43 °C - closed cup

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