11138600001
Roche
Interleukin-6, human (hIL-6)
recombinant (E. coli)
Synonym(s):
hIL-6, interleukin-6 human
About This Item
Recommended Products
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
>98% (SDS-PAGE)
form
solution
potency
<0.01 ng/mL EC50
mol wt
20,600 Da
packaging
pkg of 200,000 U (2 μg, 1 ml)
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | hybridoma: suitable
impurities
<0.1 EU/μgtested (LAL test)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IL6(3569)
General description
Specificity
IL-6, a 26 kD protein, also known as B-cell stimulatory factor 2 (BSF-2, BCDF), interferon-β2 (IFN-β2), hybridoma/plasmacytoma growth factor (H(P)GF), and hepatocyte-stimulating factor is produced by many cell types (e.g., T-cells, monocytes, fibroblasts, endothelial cells). IL-6 induces the terminal maturation of activated B-cells into antibody-producing cells (B-cell differentiation activity. The hepatic acute phase response is induced by IL-6 (hepatocyte-stimulating activity). It was shown that IL-6 and IL-3 act synergistically to support the proliferation of hematopoietic stem cells (hemapoietic activity), however, IL-6 does not have antiviral activity, although it has been called IFN-β2. High affinity receptors for IL-6 are found on many cell types.
Application
Biochem/physiol Actions
Quality
Note: 1 EU corresponds to 0.1 ng
Sequence
Unit Definition
Physical form
Preparation Note
10 to 100 U/ml (0.1 to 1 ng/ml)
A concentration of 100 U/ml (1 ng/ml) is recommended for the preparation of B-cell hybridomas
Working solution: Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution
Analysis Note
Other Notes
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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