MilliporeSigma performs mammalian cell culture without antibiotics unless required.
For SCC252, there are no documented issues with using pen/strep, and so the decision to use antibiotics to prevent contamination should be based on the individual researcher's needs and experience.
SCC252
CH12.LX Mouse Lymphoma Cell Line
CH12.LX mouse lymphoma cell line is ideal for studies of B-cell activation and expansion.
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Pricing and availability is not currently available.
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application(s)
cell analysis
General description
Naïve B-cells differentiate and expand upon antigenic stimulation, undergoing class-switch recombination of immunoglobulin genes and forming antibody-producing plasma cells and memory cells that generate upon recall stimulation (1). B-cell models are useful for elucidating pathways of differentiation and proliferation mechanisms of lymphoma.
The CH12.LX mouse lymphoma cell line is an established and well-characterized B-cell model. CH12.LX cells express Ly-12 and have the characteristic IgM+CD5+CD23-cell surface marker expression of B1a B lymphocytes, as well as expressing CD19, CD11b, MHC II and alpha-4-integrin (3). CH12.LX cells are responsive to a variety of antigens including lipopolysaccharide, expressing the activation protein CD14 (4). CH12.LX cells proliferate rapidly and are ideal for studies of B-cell activation and expansion.
<bold>Source:</bold>
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes (2).
Research Category:
Cancer
The CH12.LX mouse lymphoma cell line is an established and well-characterized B-cell model. CH12.LX cells express Ly-12 and have the characteristic IgM+CD5+CD23-cell surface marker expression of B1a B lymphocytes, as well as expressing CD19, CD11b, MHC II and alpha-4-integrin (3). CH12.LX cells are responsive to a variety of antigens including lipopolysaccharide, expressing the activation protein CD14 (4). CH12.LX cells proliferate rapidly and are ideal for studies of B-cell activation and expansion.
<bold>Source:</bold>
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes (2).
Research Category:
Cancer
Cell Line Origin
Mouse, Cancer Cells
Packaging
≥1X106 cells/vial
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact [email protected].
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hi, I will use the CH12.LX cell line, can add to the thawing and expansion medium 1X Penicillin/Streptomycin?
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