SCC066

Blood-Brain Barrier hCMEC/D3 Cell Line

Human

• Synonym(s): Human BBB cell line, Human blood-brain barrier cell line, Human cerebral endothelial cell line, CMEC/D3 cell Line, hCMEC/D3 cell line
• UNSPSC Code: 41106514
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: human

Properties

• Product Name: Blood-Brain Barrier hCMEC/D3 Cell Line, The hCMEC/D3 BBB cell line has been extensively characterized for brain endothelial phenotype and is a model of human blood-brain barrier (BBB) function.
• biological source: human
• Quality Segment: 100
• technique(s): cell culture | mammalian: suitable, drug transporter assay: suitable
• shipped in: liquid nitrogen
• storage temp.: -140 to -196°C

Description

General description

Listen to an Audio Overview
The blood–brain barrier (BBB) is a highly selective permeability barrier that separates the circulating blood from the brain extracellular fluid in the central nervous system. The blood–brain barrier is formed by capillary endothelial cells, which are connected by tight junctions with an extremely high electrical resistivity. The blood–brain barrier allows the passage of water, some gases, and lipid soluble molecules by passive diffusion, as well as the selective transport of molecules such as glucose and amino acids that are crucial to neural function. On the other hand, the blood–brain barrier may prevent the entry of lipophilic, potential neurotoxins by way of an active transport mechanism mediated by P-glycoprotein. Astrocytes are necessary to create the blood–brain barrier. The blood–brain barrier (BBB) prevents entry into the brain of most drugs from the blood. The presence of the BBB makes difficult the development of new treatments of brain diseases, or new radiopharmaceuticals for neuroimaging of brain.

The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS).

Reference:
Couraud PO, et al. (2008) The human brain endothelial cell line hCMEC/D3 as a human blood‐brain barrier model for drug transport studies. Fluids Barriers CNS. 2013 Mar 26;10(1):16.

Application

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

The hCMEC/D3 BBB cell line has been extensively characterized for brain endothelial phenotype and is a model of human blood-brain barrier (BBB) function.

Biochem/physiol Actions

Endothelial Cells

Preparation Note

Blood-Brain Barrier hCMEC/D3 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable