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ECM551

Sigma-Aldrich

QCM Collagen Cell Invasion Assay, 24-well (8 µm), Colorimetric

The Cell Invasion Assay Kit kuses a 24 well plate with 8 um pores, which is ideal for evaluation of invasive tumor cells.

Synonym(s):

Cell invasion kit

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.32

Quality Level

species reactivity

vertebrates

manufacturer/tradename

Chemicon®
QCM

technique(s)

cell based assay: suitable

detection method

colorimetric

shipped in

wet ice

Application

Research Category
Cell Structure
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 24 samples. The quantitative nature of this assay is especially useful for screening of pharmacological agents.

The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

Packaging

24 wells

Components

Sterile 24-well Cell Invasion Plate Assembly: (Part No. 90248) Two 24-well plates with 12 collagen-coated inserts per plate (24 inserts total/kit).

Cell Stain: (Part No. 90144) One 20 mL bottle.

Extraction Buffer: (Part No. 90145) One 20 mL bottle.

Cotton Swabs: (Part No. 10202) 50 each.

Forceps: (Part No. 10203) One each.

Storage and Stability

Store kit materials at 2-8°C for up to their expiration date. Do not freeze.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

53.6 °F

Flash Point(C)

12 °C


Certificates of Analysis (COA)

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Use of a reconstituted basement membrane to measure cell invasiveness and select for highly invasive tumor cells.
Terranova, V P, et al.
Proceedings of the National Academy of Sciences of the USA, 83, 465-469 (1986)
A new in vitro assay for quantitating tumor cell invasion.
Repesh, L A
Invasion & Metastasis, 9, 192-208 (1989)
MiR-145 inhibits tumor angiogenesis and growth by N-RAS and VEGF.
Chao Zou,Qing Xu,Feng Mao,Dan Li,Chuanxiu Bian,Ling-Zhi Liu,Yue Jiang,Xiaona Chen et al.
Cell Cycle null
A rapid in vitro assay for quantitating the invasive potential of tumor cells.
Albini, A, et al.
Cancer Research, 47, 3239-3245 (1987)
Cong Guo et al.
Oncotarget, 6(33), 34402-34413 (2015-09-30)
Gpbar1 (TGR5), a membrane-bound bile acid receptor, is well known for its roles in regulation of energy homeostasis and glucose metabolism. Here we show that TGR5 is a suppressor of gastric cancer cell proliferation and migration through antagonizing STAT3 signaling

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