AP308P

Goat Anti-Mouse IgG Antibody, (H+L) HRP conjugate

1 mg/mL, Chemicon^®

• Synonym(s): HRP-Conjugated Goat Anti-Mouse IgG
• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA, ICC, IHC, WB
• Citations: 193

Properties

• biological source: goat
• Quality Segment: 100
• conjugate: peroxidase conjugate
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.

Application

ELISA:
1:3,000 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Immunohistochemistry/immunocytochemistry:
1:200-1:500 dilution can be used (Bullock, G.R., 1982; Javois, L.C., 1994).

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

This Goat anti-Mouse IgG Antibody, (H+L) HRP conjugate is validated for use in ELISA, IC, IH, WB for the detection of Mouse IgG.

Biochem/physiol Actions

Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.

Physical form

Affinity purified

Purified Goat IgG conjugated to horseradish peroxidase in buffer containing liquid in 0.01M PBS, pH 7.1 with 15mg/mL BSA and 0.1% preservative.

Preparation Note

The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE.

Analysis Note

Western Blot, chromogenic:
1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Western Blot, chemiluminescent:
1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable