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407709

Insulin, Zinc, Human, Recombinant, P. pastoris

Synonym(s):

Insulin, Zinc, Human, Recombinant, P. pastoris

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
Biological source:
yeast (Pichia pastoris)
Form:
solid
Assay:
≥95—105%
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biological source

yeast (Pichia pastoris)

Quality Level

description

Merck USA index - 14, 4980

sterility

non-sterile

assay

≥95—105%

form

solid

specific activity

≥25 IU/mg (dry basis)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

<10 EU/mg Endotoxin (EU/mg protein)

color

white to off-white

solubility

0.01 M HCl: soluble

shipped in

wet ice

storage temp.

−20°C

InChI key

PBGKTOXHQIOBKM-FHFVDXKLSA-N

General description

Recombinant, human insulin (zinc) expressed in P. pastoris. Peptide anabolic hormone produced by pancreatic β-cells. Increases the synthesis of protein, fat, glycogen, and substrate oxidation in mitochondria. Interacts with membrane insulin receptor to cause its biological actions.

Preparation Note

Following reconstitution store in the refrigerator (4°C). Stock solutions are stable for up to 6 weeks at 4°C.
Insulin is practically insoluble from pH 4.5-7.0.

Other Notes

Mohan, C., et al. 1989. Curr. Topics Cell. Reg.30, 105.
Johnson, I.S. 1983. Science219, 632.
≥25 IU/mg (dry basis).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
11376497001I35361342106
biological source

yeast (Pichia pastoris)

biological source

-

biological source

Saccharomyces cerevisiae

biological source

-

assay

≥95—105%

assay

>98% (SDS-PAGE)

assay

-

assay

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

-

form

solid

form

lyophilized (clear, colorless solution after reconstitution)

form

lyophilized powder

form

solid

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

shipped in

wet ice

shipped in

-

shipped in

ambient

shipped in

-


Storage Class

11 - Combustible Solids

wgk

nwg



Certificates of Analysis (COA)

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Mohan Sreelekshmi et al.
Journal of biochemical and molecular toxicology, 35(6), 1-8 (2021-03-03)
Glucokinase (GK), a key regulator of hepatic glucose metabolism in the liver and glucose sensor and mediator in the secretion of insulin in the pancreas, is not studied in detail for its therapeutic application in diabetes. Herein, we study the
Kapil Manglani et al.
Cell biology international, 46(3), 488-497 (2021-12-06)
Role of CDK5 and its inhibition in various neuronal processes and functions are well established. However, role of CDK5 and its inhibition in neuronal insulin-signaling and-resistance is not yet explored. In the present study, we investigated the effect of CDK5
Medha Sharma et al.
Cell communication and signaling : CCS, 20(1), 179-179 (2022-11-16)
The aim of the present study was to determine the role of individual PHLPP isoforms in insulin signaling and insulin resistance in neuronal cells. PHLPP isoforms were either silenced or overexpressed individually, and the effects were observed on individual Akt



Global Trade Item Number

SKUGTIN
407709-50MG07790788050047

Questions

  1. I want know about the MW and purity of this product

    1 answer
    1. The molecular weight of Product 407709 Insulin, Zinc, Human, Recombinant, is 5807 Da. Purity is 99.2 % pure by HPLC, < 10.0 EnU/mg, and Potency of 28.5 IU/mg.

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