The product is supplied as a 1mg/mL of solution in 0.05 M HEPES and 0.1 M Na2SO4 with preservative. In Western blotting, typical working dilutions may range from 2 - 5 µg/mL. Effective working concentrations may vary and be as low as 0.1 µg/mL End-user optimization is recommended.
18-152
Streptavidin, HRP conjugate
1 mg Streptavidin, HRP conjugate for ELISA, Western Blotting, ICC & IHC.
Synonym(s):
detection reagent
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About This Item
UNSPSC Code:
12352204
NACRES:
NB.22
eCl@ss:
32160405
Pricing and availability is not currently available.
Quality Segment
form
liquid
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunodetection: suitable, immunohistochemistry: suitable, western blot: suitable
shipped in
wet ice
Application
Streptavidin, horseradish peroxidase (HRP) conjugate has been used in:
- cell-based enzyme linked immunosorbent assay (ELISA)
- sandwich ELISA
- microneutralization assay
Biochem/physiol Actions
Streptavidin antibody, covalently bound to horseradish peroxidase (HRP) is used in enzyme-linked detection methods. The conjugated enzyme catalyzes the breakdown of certain substrates, leading to signal generation.
Physical form
0.05M HEPES, 0.1M Na2SO4, pH6.8, preservative.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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