1.12018

Albumin fraction V

(from bovine serum) for biochemistry

• Synonym(s): Albumin fraction V, Bovine serum albumin, Serum albumin
• CAS Number: 90604-29-8
• MDL number: MFCD00000745
• UNSPSC Code: 12352202
• EC Index Number: 292-322-5
• NACRES: NA.21

Properties

• biological source: bovine
• form: solid
• concentration: ≥97% protein (spectralphotometric; calc. on anhydrous substance); ≥98% (Assay of Albumin (Agarose gel electrophoresis))
• technique(s): immunoassay: suitable; western blot: suitable
• pH: 6.8-7.2 (20 °C, 1 g/L in H₂O)
• solubility: 1 g/L
• bulk density: 150 kg/m³
• storage temp.: 2-8°C

Description

General description

Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.

Application

Albumin fraction V has been used:

• as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
• as a blocking agent in western blot analysis
• as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids. 

Analysis Note

Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable