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1.10649

Bacillus subtilis (BGA) spore suspension

Indicator organism for the detection of antimicrobial inhibitors.

Synonym(s):

B. subtilis, Biological indicators

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About This Item

NACRES:
NA.84
UNSPSC Code:
41116130
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Quality Segment

100

form

liquid

technique(s)

microbiological culture: suitable

density

1 g/cm3

application(s)

detection
food and beverages
microbiology

storage temp.

2-8°C

suitability

Bacillus spp.

General description

Bacillus subtilis (BGA) spore suspension is used for the detection of antimicrobial inhibitors. The antimicrobial inhibitors test is conducted as an agar diffusion test. The spores of Bacillus subtilis (BGA) are the test organisms. The inhibitors inhibit the growth of the test bacteria. The inhibition of growth is indicated by a zone of inhibition measured between the edge of the piece of tissue and the limit of growth. Complete inhibition of growth with an inhibition zone of a minimum of 2 mm can be regarded as a positive result, and an inhibition zone of 1 to 2 mm as a dubious result, if the parallel controls have inhibition zones of approx. 6 mm.

Application

Bacillus subtilis (BGA) spore suspension is used for the detection of antimicrobial inhibitors in meat products by routine methods. BGA spore suspension is used along with the culture media test agar pH 6.0 (1.10663) and test agar pH 8.0 (1.10664) for the inhibitor test.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–3 of 3 Questions  

  1. Are the cells in living condition and how long are they viable in the suspension if stored at appropriate conditions? Do the cells need to be activated or grown separately or can they be directly utilised ?

    1 answer

    1. Product contains ampoules with spores of Bacillus subtilius, no vegetative cells. The expiration date of the product is on the label of the product box; the shelf life of the product is 17 months when stored at the given storage temperature.


      The spores can directly be added to the melted agar, with an agar temperature of not more than 50°C. Please consult the technical data sheet of the product: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/299/903/110649-bacillus-subtilis-bga-spore-suspension-for-the-inhibitor-test-ms.pdf

      Helpful?

  2. In what medium are the spores suspended? How have these spores been stored?

    1 answer

    1. Unfortunately, the buffer formulation used to prepare the spores remains proprietary.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/691/09-102-1-pb-110649-engl.pdf

      Helpful?

  3. Can I know the concentration of the suspension solutions?

    1 answer

    1. Product 110649MM, Bacillus subtilis (BGA) spore suspension, contains viable spores of Bacillus subtilis DSM 618 at a concentration of 8E+06 - 5E+07 CFU/mL.

      Helpful?

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