1.00213
NutriSelect® prime Sorbitol MacConkey (SMAC) Agar
acc. FDA-BAM, ISO 16654
Synonym(s):
CT-SMAC Agar, MacConkey Agar with Sorbitol, SMAC Agar
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About This Item
NACRES:
NA.74
UNSPSC Code:
41106213
Technique(s):
microbiological culture: suitable
Storage temp.:
15-25°C
Shipped in:
ambient
Pricing and availability is not currently available.
agency
FDA-BAM
Quality Segment
form
powder
shelf life
5 yr
composition
- Enzymatic digest of casein 17.0 g/L
- Enzymatic digest of animal tissue 3.0 g/L
- Sodium chloride 5.0 g/L
- Bile salts No. 3 1.5 g/L
- Sorbitol 10.0 g/L
- Neutral red 0.03 g/L
- Crystal violet 0.001 g/L
- Agar-agar 15.0 g/L
packaging
container of 500 g
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
7.1±0.2 (25 °C)
solubility
water: soluble
application(s)
food and beverages
pharmaceutical
compatibility
for use with CT supplement (77981)
shipped in
ambient
storage temp.
15-25°C
suitability
selective and differential for Escherichia 0157:H7
General description
GranuCult® prime Sorbitol MacConkey (SMAC) Agar is a selective agar medium to isolate and differentiate enterohemorrhagic (EHEC) E. coli 0157 from food, animal feed and other samples.
The presence of bile salts and crystal violet inhibits the growth of gram-positive microbes thereby enhancing the growth of gram-negative microorganisms.
The specific selectivity of the medium is done by the addition of Cefixime potassium Tellurite supplement towards E. coli 0157:H7 and in turn suppresses the accompanying flora. Addition of sorbitol in the medium along with pH indicator neutral red is used to detect the sorbitol positive colonies which appear red in color. Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Enzymatic digest of casein and animal tissue provides nitrogen, vitamins, and amino acids to the growing microbes. Agar is the solidifying agent.
The presence of bile salts and crystal violet inhibits the growth of gram-positive microbes thereby enhancing the growth of gram-negative microorganisms.
The specific selectivity of the medium is done by the addition of Cefixime potassium Tellurite supplement towards E. coli 0157:H7 and in turn suppresses the accompanying flora. Addition of sorbitol in the medium along with pH indicator neutral red is used to detect the sorbitol positive colonies which appear red in color. Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Enzymatic digest of casein and animal tissue provides nitrogen, vitamins, and amino acids to the growing microbes. Agar is the solidifying agent.
Application
GranuCult® prime Sorbitol MacConkey (SMAC) Agar is used for the isolation and differentiation of sorbitol negative Escherichia coli serotype O157 from food, animal feed and other test samples.
Analysis Note
- The dehydrated media is granulated with light pink color.
- Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
WGK
WGK 3
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
548-62-9
CAS No.
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