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Supelco

SUPELCOSIL LC-NH₂ NP (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

SUPELCOSIL LC-NH2-NP 5UM 25CMX4.6MM HPLC COLUMN

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

Product Name

SUPELCOSIL LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L8

Quality Level

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

3% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 5.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

pH

2-7.5

application(s)

food and beverages

separation technique

normal phase

General description

An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.

These columns should be used with non-aqueous mobile phases only.
SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.

Features and Benefits

• show stable retention in normal-phase separations
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
SUPELCOSIL LC-NH2-NP columns:
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Sainath Babu et al.
Toxicology mechanisms and methods, 25(5), 410-416 (2015-05-30)
We have developed a simple, reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of bisphenol A (BPA) in thermal paper cash register receipts (CRs). The method is suitable for analysis of other types of bisphenols and it involves an
R Herráez-Hernández et al.
Journal of chromatographic science, 35(4), 169-175 (1997-04-01)
A chromatographic method for the analysis of amphetamine and related compounds in urine using 3,5-dinitrobenzoyl chloride (3,5-DNB) as a labeling reagent is presented. This assay is based on the employment of solid-phase extraction (SPE) cartridges for sample cleanup and derivatization.
F N Hussain et al.
Journal of chromatography. B, Biomedical sciences and applications, 716(1-2), 257-266 (1998-11-21)
We describe a sensitive high-performance liquid chromatographic method for the determination of 5-aminosalicylic acid and N-acetyl-5-aminosalicylic acid in rectal tissue biopsies. Samples were derivatised using propionic anhydride and proteins were precipitated with methanol. A Supelcosil ABZ column (150x4.6 mm I.D.
S Moret et al.
Zeitschrift fur Lebensmittel-Untersuchung und -Forschung, 201(4), 322-326 (1995-10-01)
This paper proposes a simple HPLC method for the determination of polycyclic aromatic hydrocarbons (PAHs) in water, wine and beer. Samples were purified by PAH collection in solid-phase extraction (SPE) and analysed by reversed-phase HPLC (Supelcosil LC-PAH column from Supelco).
Thadikamala Sathish et al.
Pakistan journal of pharmaceutical sciences, 22(1), 68-73 (2009-01-27)
An accurate, simple, reproducible and sensitive RP-HPLC method for the determination of bharangin has been developed and validated. The separation of bharangin and 2-nitroaniline (internal standard) was achieved on Supelcosil LC-18 (3micro, 150 x 4.6 mm i.d.) column using UV

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