MAK159
Mitochondrial Membrane Potential Kit
sufficient for 500 fluorometric tests (microplate readers)
Synonym(s):
JC-10 Assay, JC-10 Mitochondrial Membrane Potential Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.25
Recommended Products
usage
sufficient for 500 fluorometric tests (microplate readers)
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
Application
Mitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential.
Suitability
This kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.
Principle
This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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