I3146
ITS Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Human ITS Liquid Media, Insulin-transferrin-sodium selenite
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About This Item
UNSPSC Code:
12352205
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
Quality Level
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
Application
ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
- α mouse liver 12 (AML-12) cells
- rat fetal testis explants
- human dental pulp stem cells (DPSC)
Biochem/physiol Actions
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
- Transferrin: an iron-transport protein.
- Selenium: an essential trace element normally provided by serum.
Components
Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
Other Notes
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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