G9637
Glycerol 3-phosphate Oxidase from Pediococcus sp.
lyophilized powder, 40-80 units/mg solid, pH 8.1
Synonym(s):
sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase
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About This Item
Recommended Products
biological source
bacterial (Pediococcus spp.)
Quality Level
form
lyophilized powder
specific activity
40-80 units/mg solid, pH 8.1
mol wt
~76 kDa (gel filtration)
storage temp.
2-8°C
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General description
Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.
Application
Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode.
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.
Physical properties
Isoelectric point : 4.1 -/+0.1
Michaelis constants : 3.2x10 -3M (L-α-Glycerophosphate), 6.8 x 10 -3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg++, Ag+
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40oC
pH Stability : pH 6.5 - 8.5 (25oC, 20hr) Thermal stability : below 40oC (pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate
Michaelis constants : 3.2x10 -3M (L-α-Glycerophosphate), 6.8 x 10 -3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg++, Ag+
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40oC
pH Stability : pH 6.5 - 8.5 (25oC, 20hr) Thermal stability : below 40oC (pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate
Unit Definition
One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at 37°C, at the appropriate pH.
Physical form
Lyophilized powder containing stabilizers
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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