D0426
DAB with Metal Enhancer
SIGMAFAST™, peroxidase substrate, tablet
Synonym(s):
3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name
SIGMAFAST™ DAB with Metal Enhancer, tablet
form
tablet
Quality Level
solubility
water: 1 tablet/5 mL
storage temp.
−20°C
General description
SIGMA FAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Application
SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry, dorsal root ganglion (DRG) neurons and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
Reconstitution
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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