56822
Immersion oil
for microscopy
Synonym(s):
immersion medium for light microscopy
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About This Item
Recommended Products
biological source
synthetic
Quality Level
grade
for microscopy
form
liquid
refractive index
n20/D 1.516
viscosity
100-120 mPa.s(20 °C)
density
1.025 g/mL at 20 °C
application(s)
hematology
histology
storage temp.
room temp
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General description
Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.
Application
Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.
Principle
Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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