Skip to Content
Merck
All Photos(1)

Key Documents

G005682

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)
mobile phase: [A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B)
flow rate: 0.6 mL/min
column temp.: 25 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 2 μL
sample: 1000 μg/mL in 10:90, water: acetonitrile

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Caffeine
    Taurine

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service