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G003761

HPLC Analysis of Amphetamines in Urine on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and Standard C18

application for SPE, application for HPLC

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About This Item

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: human urine spiked amphetamine and methylamphetamine at with 2 mg/mL
SPE tube/cartridge: Discovery DSC-MCAX, 100 mg/3mL (52783-U)
condition: DSC-MCAX SPE tube: 1 mL methanol; 1 mL 50 mM ammonium acetate, pH 6.0; C-18 SPE tube: 1 mL methanol; 1 mL DI water
sample addition: 1 mL
washing: DSC-MCAX SPE tube: 1 mL 50 mM ammonium acetate, pH 6.0; 1 mL 1M acetic acid; 1mL methanol; C-18 SPE tube: 1 mL DI water; 1 mL 20% methanol
elution: DSC-MCAX SPE tube: 1 mL 5% ammonium hydroxide in methanol; C-18 SPE tube: 1 mL methanol
eluate post-treatment: evaporate to dryness with nitrogen at room temperature, reconstitute in mobile phase
column: Discovery HS F5, 15 cm x 4.6 mm I.D., 5 μm particles (567516-U)
mobile phase: [A] 10 mM ammonium acetate, pH 4.5: [B] methanol; (35:65, A:B)
flow rate: 2 mL/min
column temp.: 40 °C
detector: UV, 210 nm
injection: 10 μL

suitability

application for HPLC
application for SPE

application(s)

forensics and toxicology

Application

Sample Pre-Treatment:
1 mL human urine was spiked with 2 mg/mL amphetamine and methylamphetamine. The spiked sample was diluted 1:1 with 1:1 with 50 mM ammonium acetate, pH 6.0.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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