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MAK067

Sigma-Aldrich

Malate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

L-Malate is a tricarboxylic acid cycle intermediate and a critical component of the malate-aspartate shuttle. The Malate-aspartate shuttle is required for transporting reducing equivalents across the mitochondrial membrane for use in oxidative phosphorylation. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malate is important in the manufacturing of beer, wine, cheese, and fruits. The Malate Assay kit is an easy and sensitive assay to measure the malate level in a variety of samples.

In this kit, malate is oxidized to generate a product specifically detected by colorimetric (450 nm) assay. Typical sensitivities of detection for this kit are 1-35 nmol of malate.

Application

Malate Assay Kit has been used to determine the concentration of malate in samples.

Suitability

Suitable for the measurement of L(-) Malate level in a variety of samples including tissue, cells and food and beverages (for example beer, wine, cheese and fruit)

Principle

Malate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the malate present. Typical detection range for this kit is 5–25 nmoles of malate.

replaced by

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

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Stephen R Thom et al.
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We hypothesized that elevations of carbon dioxide (CO2) commonly found in modern buildings will stimulate leukocytes to produce microparticles (MPs) and activate the nucleotide-binding domain-like receptor 3 (NLRP3) inflammasome due to mitochondrial oxidative stress. Human and murine neutrophils generate MPs
Michael W Shane et al.
Plant physiology, 161(4), 1634-1644 (2013-02-15)
Accumulating evidence indicates important functions for phosphoenolpyruvate (PEP) carboxylase (PEPC) in inorganic phosphate (Pi)-starved plants. This includes controlling the production of organic acid anions (malate, citrate) that are excreted in copious amounts by proteoid roots of nonmycorrhizal species such as
Melatonin alleviates adipose inflammation through elevating α-ketoglutarate and diverting adipose-derived exosomes to macrophages in mice.
Liu Z, et al.
Journal of Pineal Research, 64(1), e12455-e12455 (2018)
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Nature metabolism, 3(1), 90-106 (2021-01-06)
Organelles use specialized molecules to regulate their essential cellular processes. However, systematically elucidating the subcellular distribution and function of molecules such as long non-coding RNAs (lncRNAs) in cellular homeostasis and diseases has not been fully achieved. Here, we reveal the
Melatonin alleviates adipose inflammation through elevating α-ketoglutarate and diverting adipose-derived exosomes to macrophages in mice
Liu Z, et al.
Journal of Pineal Research, 64(1), e12455-e12455 (2018)
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