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H0537

Millipore

HIS-Select® HF Nickel Affinity Gel

Synonym(s):

Ni-NTA HF resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

form

suspension

Quality Level

200

technique(s)

affinity chromatography: suitable

matrix

Highly cross-linked 6% Beaded Agarose

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

storage temp.

2-8°C

General description

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Application

Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of His-tag® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Features and Benefits

  • High selectivity for higher purity
  • Unique non-charged hydrophilic linkage reduced non-specific binding
  • Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
  • Highly cross-linked agarose matrix allows for purification under higher pressures
  • Binding under denaturing or non-denaturing conditions
  • One-step purification

Physical form

1:1 suspension in a 30% ethanol solution

Preparation Note

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Legal Information

FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2

Target Organs

Eyes,Central nervous system, Respiratory Tract

WGK

WGK 3

Flash Point(F)

88.0 °F

Flash Point(C)

31.1 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Minoru Tanaka et al.
Nature chemical biology, 12(12), 1089-1096 (2016-10-25)
Cellular signaling is often propagated by multivalent interactions. Multivalency creates avidity, allowing stable biophysical recognition. Multivalency is an attractive strategy for achieving potent binding to protein targets, as the affinity of bivalent ligands is often greater than the sum of
David S Pitcher et al.
EBioMedicine, 2(7), 642-648 (2015-08-20)
The proteasome inhibitor Bortezomib is used to treat multiple myeloma (MM). Bortezomib inhibits protein degradation by inactivating proteasomes' active-sites. MM cells are exquisitely sensitive to Bortezomib - exhibiting a low-nanomolar IC(50) - suggesting that minimal inhibition of degradation suffices to
Obtaining of Agr2 Specific Antibodies and Determination of the Agr2 Protein Distribution Pattern during Early Embryonic Development and Tadpole Regeneration in Xenopus laevis
Ivanova, A.S. et al.
Russian journal of developmental biology, 49, 393-397 (2018)
Irving E Vega et al.
Frontiers in neuroscience, 12, 879-879 (2018-12-19)
Aggregation of the protein tau is a pathological hallmark of Alzheimer's disease (AD) and related disorders. However, the molecular mechanisms that lead to tau protein aggregation are still unclear. Previously, we showed that EFhd2 protein is associated with pathological aggregated
Jian Ming Khor et al.
Development (Cambridge, England), 146(16) (2019-07-25)
Alx1 is a conserved regulator of skeletogenesis in echinoderms and evolutionary changes in Alx1 sequence and expression have played a pivotal role in modifying programs of skeletogenesis within the phylum. Alx1 regulates a large suite of effector genes that control
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