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G4048

Sigma-Aldrich

Anti-GLUT4 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Glucose transporter 4, Anti-SLC2A4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

mouse, human

concentration

~1.5 mg/mL

technique(s)

indirect immunofluorescence: 15-20 μg/mL using C2C12 cells
western blot: 1-2 μg/mL using C2C12 cell lysate and HepG2 cell lysate

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SLC2A4(6517)
mouse ... Slc2a4(20528)
rat ... Slc2a4(25139)

Related Categories

General description

The GLUT4 (glucose transporter 4) gene, also known as SLC2A4 (solute carrier family 2 member 4) is mapped to human chromosome 17p13.1.The expression of GLUT4 (glucose transporter 4) is the highest in skeletal and adipose tissue.

Application

Anti-GLUT4 (C-terminal) antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml in whole cell lysate of C2C12 and HepG2 cells. A working dilution of 1:3000 was used for immunoblotting in whole cell lysate of HEK-293 cells. . Anti-GLUT4 (C-terminal) antibody has also been used for immunoblotting in CHO-K1 cells. Antibody concentration of 15-20 μg/ml is recommended for immunofluorescence in C2C12 cells.
Anti-GLUT4 (C-terminal) antibody produced in rabbit has been used in western blotting and immunofluorescence assay.

Biochem/physiol Actions

GLUT4 is an insulin-regulated glucose transporter that facilitates the uptake of glusose by fat and muscle cells. Generally restricted to storage vesicles, GLUT4 translocates to the plasma membrane in response to insulin stimulation. The vital function of GLUT4 is regulation of glucose utilization by the cells. Following meal consumption, insulin secreted by the pancreas binds to receptors on the muscle and adipose and activates the PI3K-Akt pathway. Activation of this pathway triggers the secretion of GLUT4 from the vesicles that translocate to the plasma membrane. An overall decrease in the expression of GLUT4 results in diabetes and a selective disruption of GLUT4, in skeletal or adipose tissue, results in insulin resistance

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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d-Opioid receptors stimulate GLUT1-mediated glucose uptake through Src- and IGF-1 receptor-dependent activation of PI3-kinase signalling in CHO cells
Olianas MC et al
The British Journal of Ophthalmology, 163, 624-637 (2011)
Stephan Nieuwoudt et al.
American journal of physiology. Cell physiology, 313(5), C575-C583 (2017-08-25)
We are interested in understanding mechanisms that govern the protective role of exercise against lipid-induced insulin resistance, a key driver of type 2 diabetes. In this context, cell culture models provide a level of abstraction that aid in our understanding
Expression of Caveolin 1 is enhanced by DNA demethylation during adipocyte differentiation. status of insulin signaling
Palacios OS, et al.
Testing, 9(4), e95100-e95100 (2014)
Nolan J Hoffman et al.
Trends in endocrinology and metabolism: TEM, 22(3), 110-116 (2011-01-11)
Solving how insulin regulates glucose transport into skeletal muscle and adipose tissue remains a fundamental challenge in biology and a significant issue in medicine. A central feature of this process is the coordinated accumulation of the glucose transporter GLUT4 into
Signaling, cytoskeletal and membrane mechanisms regulating GLUT4 exocytosis
Hoffman NJ and Elmendorf JS
Trends in Endocrinology and Metabolism, 22(3), 110-116 (2011)

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