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F2648

Sigma-Aldrich

Monoclonal Anti-CD11b−FITC antibody produced in mouse

clone ICRF44, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-CD11b

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ICRF44, monoclonal

form

buffered aqueous solution

mol wt

antigen 165-170 kDa

species reactivity

human

storage condition

protect from light

technique(s)

flow cytometry: 10 μL using 1 × 106 cells

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ITGAM(3684)

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General description

CD11b is an α/β heterodimeric glycoprotein. It belongs to the β2 integrin family and also referred as Mac-1, CR3, MO-1, and C3bi receptor.
Monoclonal Anti-Human CD11b antibody (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with the human monocytes. CD11b is expressed on the surface of circulating monocytes, granulocytes, and certain natural killer (NK) cells. It is also present on subsets of macrophages. CD11b is present in subcellular granules and is translocated to the surface after activation.

Specificity

Recognizes the CD11b α-chain of the human CD11b/CD18 complex. The antibody is useful for studies on cell adhesion and migration and for the detection of leukocyte adhesion deficiencies. It may be used to stain acetone-fixed cryostat sections or cell preparations. The epitope recognized by the antibody is formalin sensitive.
2nd Workshop: code no. M104
3rd Workshop: code no. 202 and 238
4th Workshop: code no. M47

Immunogen

human monocytes.

Application

Anti-CD11b-FITC antibody is suitable for flow cytometric analysis of CD11b expression in RA-induced differentiation of human promyelocytic leukemia cells. It is also suitable for flow cytometric analysis of spleen cells to study the beneficial effects of dietary consumption of n-3 polyunsaturated fatty acids (PUFA) and two selective estrogen receptor modulator (SERM) derivatives.

Biochem/physiol Actions

Anti-CD11b-FITC antibody is expressed on the surface of circulating monocytes, granulocytes, and certain NK cells. It is also present on subsets of macrophages and in subcellular granules of granulocytes. Upon activation, it translocates to the surface. It forms a CD11b/CD18 complex to participate in a variety of cell-cell and cell-substrate interactions such as attachment and phagocytosis of particles coated with C3bi by granulocytes and macrophages and phagocytosis of opsonized pathogens. It also plays a role in the initiation of a coagulation protease cascade and in cell migration mechanisms.
Surface expression of cluster of differentiation molecule CD11b/CD18 is capable of both functional and quantitative upregulation. CD11b/CD18 functions as a receptor for C3bi, clotting factor X, fibrinogen, and intercellular adhesion molecule 1 (ICAM-1). CD11b/CD18 is involved in a variety of cell-cell and cell-substrate interactions such as attachment and phagocytosis of particles coated with complement receptor type 3 (C3bi) by granulocytes and macrophages and phagocytosis of opsonized pathogens. It also plays a role in the initiation of a coagulation protease cascade and in cell migration mechanisms. The endothelial cell counter-receptor for CD11b/CD18 is ICAM-1. Monoclonal Anti-Human CD11b can be used to stain acetone-fixed cryostat sections or cell preparations. The epitope recognized by the antibody is formalin sensitive.

Target description

CD11b α-chain of the human CD11b/CD18 complex is an α/β heterodimeric glycoprotein which belongs to the β2 integrin family. It is also known as Mac-1, CR3, MO-1 and C3bi receptor. CD11b is expressed on the surface of circulating monocytes, granulocytes and certain NK cells and is also present in subsets of macrophages. It functions as a receptor for iC3b, clotting factor X, fibrinogen and ICAM-1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L Zeitlin et al.
Journal of cellular biochemistry, 90(2), 347-360 (2003-09-25)
We studied the beneficial effects of dietary consumption of n-3 polyunsaturated fatty acids (PUFA) and two selective estrogen receptor modulator (SERM) derivatives (SERM-I and SERM-II) and their combined effect on serum lipids, skin dermis and adipose layers, bone marrow adipogenesis
Fibrinogen stimulates macrophage chemokine secretion through toll-like receptor 4
Smiley ST, et al.
Journal of immunology (Baltimore, Md. : 1950), 167(5), 2887-2894 (2001)
Francesco Silvio Pasqualini et al.
PloS one, 13(3), e0194706-e0194706 (2018-03-29)
Cardiac tissue development and pathology have been shown to depend sensitively on microenvironmental mechanical factors, such as extracellular matrix stiffness, in both in vivo and in vitro systems. We present a novel quantitative approach to assess cardiac structure and function
B L Myones et al.
The Journal of clinical investigation, 82(2), 640-651 (1988-08-01)
Previous investigations of p150,95 (CD11c), the third member of the CD18 membrane glycoprotein family that includes CR3 (Mac-1 or CD11b) and LFA-1 (CD11a), had demonstrated that solubilized p150,95 bound to iC3b-agarose in a manner similar to isolated CR3. The current
D I Beller et al.
The Journal of experimental medicine, 156(4), 1000-1009 (1982-10-01)
Anti-Mac-1 (M1/70), a rat monoclonal antibody that reacts with mouse and human macrophages, polymorphonuclear leukocytes (PMNL), and natural killer cells, selectively inhibited complement receptor-mediated rosetting by murine macrophages and human PMNL. Preincubation of macrophages with anti-Mac-1 inhibited formation of rosettes

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