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Key Documents

HTC (BUdR)

85061110, rat liver, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

HTC (BUdR), 85061110

biological source

rat liver

growth mode

Adherent

karyotype

2n = 66

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat liver hepatoma

Cell Line Description

Clone of the original HTC hepatoma (Sigma Catalogue number. 93120108) rendered resistant to 10ug/ml BUdR, which must be grown in HAT. The cells are used in enzyme induction studies.

Application

Enzymatic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1%HAT + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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