C3411

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein

≥90% (HPLC)

• Synonym(s): BCECF
• Empirical Formula (Hill Notation): C₂₇H₂₀O₁₁
• CAS Number: 85138-49-4
• Molecular Weight: 520.44
• Beilstein: 8375770
• MDL number: MFCD00036970
• UNSPSC Code: 12352200
• PubChem Substance ID: 24892612
• NACRES: NA.32

Properties

• Quality Level: 100
• Assay: ≥90% (HPLC)
• form: powder
• storage temp.: −20°C
• SMILES string: OC(=O)CCc1cc2c(OC3=CC(=O)C(CCC(O)=O)=CC3=C2c4cc(ccc4C(O)=O)C(O)=O)cc1O
• InChI: 1S/C27H20O11/c28-19-10-21-17(7-12(19)2-5-23(30)31)25(16-9-14(26(34)35)1-4-15(16)27(36)37)18-8-13(3-6-24(32)33)20(29)11-22(18)38-21/h1,4,7-11,28H,2-3,5-6H2,(H,30,31)(H,32,33)(H,34,35)(H,36,37)
• InChI key: DKROGDOAOXDINY-UHFFFAOYSA-N

Description

Application

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein has been used in a fluorescence-based screening assay, to determine the pH.

Biochem/physiol Actions

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is useful to study multidrug resistance-associated protein (MRP) transporter action within the cell. BCECF is known to interfere with drug efflux interactions.

Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves