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A8678

Sigma-Aldrich

Agar

suitable for plant cell culture

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
NACRES:
NA.21

biological source

algae (Rhodophyceae)

form

powder

technique(s)

cell culture | plant: suitable

mp

80-90 °C (1.5% in water)

transition temp

transition temp 27-37 °C (1.5%)

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar or Agar-agar is one of the major components found in the cell wall of red algae. It is a polysaccharide complex, comprising about 70% agarose and 30% agaropectin.

Application

Washed agar has been used:
  • as a supplement (0.9% w/v) to Murashige and Skoog basal medium for plant growth
  • in the preparation of cocultivation medium for Agrobacterium tumefaciens mediated transformation in maize (Zea mays) hybrid Hi-II
  • as a component in the complete medium to regenerate protoplasts of Piriformospora indica
  • as a clean agar medium to culture Synechococcus WH7803 strain

Biochem/physiol Actions

Agar is an ester of sulfuric acid in a linear galactan form, that makes it soluble in hot water and insoluble in cold. Washed agar contain less impurities and low level of nutrients.

Preparation Note

Washed

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Andrew D Millard
Methods in molecular biology (Clifton, N.J.), 501, 33-42 (2008-12-11)
Cyanophages are a group of viruses which specifically infect cyanobacteria. The cyanobacteria are predominantly aquatic phototrophic bacteria and the two dominant genera Synechococcus and Prochlorococcus contribute significantly to primary production in the oceans. Cyanophages that infect marine cyanobacteria were first
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Fungal genetics and biology : FG & B, 46(8), 543-550 (2009-04-09)
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Juan M Vega et al.
Plant cell reports, 27(2), 297-305 (2007-10-17)
High-frequency transformation of maize (Zea mays L.) using standard binary vectors is advantageous for functional genomics and other genetic engineering studies. Recent advances in Agrobacterium tumefaciens-mediated transformation of maize have made it possible for the public to transform maize using
Lawrence R Griffing
Methods in molecular biology (Clifton, N.J.), 1691, 75-102 (2017-10-19)
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Jacky M Tiang et al.
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Arylamine N-acetyltransferase-1 (NAT1) is an enzyme that catalyzes the biotransformation of arylamine and hydrazine substrates. It also has a role in the catabolism of the folate metabolite p-aminobenzoyl glutamate. Recent bioinformatics studies have correlated NAT1 expression with various cancer subtypes.
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